FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55678
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTCTTTGTGA5070.9105930529113833No Hit
CCTCAACACTGTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGAAGA3650.6555551564352168No Hit
GAAATATGGTGACCCATTTTCTTTGTGAAAACCTGGGAAAATGCAAGTGT2590.4651747548403319No Hit
GTTTATTACAACTTAAAAGGAACTTCAGTGAATTTGTTTTTATTTTTTAA2520.4526024641689716No Hit
TATATTAACAGAAAAGACTTTCCCCCTCAACACTGTACAATTTCTGAAAT2390.42925392435073095No Hit
TCTCAGTATCATTCGACTTCAATGGAAATTTATATGGACATTTTCTGTAC1790.3214914328819282No Hit
GGTATGAAGTGTGTGTTCTGTTTGCATTGAAACAATGTAATTTTGTGTCT1710.3071231006860879No Hit
ACATAATACCCATACTTTATAGAAATAAAACTGCAAACCTGGAGAATGCT1680.3017349761126477No Hit
GTATTATGTTGCTCAGTTACTCAAATGGTACTGTATTGTTTATATTTGTA1660.29814289306368763No Hit
GTTTATATTTGTACCCCAAATAACATCGTCTGTACTTTCTGTTTTCTGTA1650.2963468515392076No Hit
CCTTTAATGAATTGCAAATATATTAACAGAAAAGACTTTCCCCCTCAACA1600.28736664391680733No Hit
GTGTATATAATGTTTAATATTTGTCAGAGCATTCTCCAGGTTTGCAGTTT1580.28377456086784725No Hit
GTACTGTATTGTTTATATTTGTACCCCAAATAACATCGTCTGTACTTTCT1520.272998311720967No Hit
GTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTCTTTGTGAAAACC1500.26940622867200686No Hit
ATGTTAAGGAGATGCTTCAAAATGTCAATTGCTTTAAACTTAAATTACCT1480.2658141456230468No Hit
GAGTATAGTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTCTTTGT1420.25503789647616654No Hit
CTTTACAGGCTTCTCAGTATCATTCGACTTCAATGGAAATTTATATGGAC1400.2514458134272064No Hit
GTACATGGGAACCCTGGGAAAATTTGCAGAATCTAAGTTGATTGTAATGA1360.24426164732928626No Hit
TTATATACACAATGAGGTAAATGTACCTTGGTCTCTTGAGAGGTAATTTA1350.2424656058048062No Hit
ATCATGAACCATGTTTTGATACCCCTTTTTCACGTTGTGCCAACGGAATA1330.2388735227558461No Hit
ACACTGTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGAAGACACAA1320.23707748123136607No Hit
GTACATGGGAAATGTAAACTTCTAAAAATATGGTTAATAACATTCAACCT1300.23348539818240596No Hit
GTATTGTTTATATTTGTACCCCAAATAACATCGTCTGTACTTTCTGTTTT1290.23168935665792592No Hit
TCCATATCCTTTAATGAATTGCAAATATATTAACAGAAAAGACTTTCCCC1280.22989331513344588No Hit
CCTTAACATATGAAGAAATATCAAACACCCTATTCCGTTGGCACAACGTG1250.22450519056000573No Hit
GAATATCATGAACCATGTTTTGATACCCCTTTTTCACGTTGTGCCAACGG1140.20474873379072522No Hit
GTATCAACGCAGAGTATAGTATGAGTCTTCACTGAGAAATATGGTGACCC1110.1993606092172851No Hit
GTGTAATCTCTGCCTTTTAAGATATGTACAGAAAATGTCCATATAAATTT1110.1993606092172851No Hit
CCCTATTCCGTTGGCACAACGTGAAAAAGGGGTATCAAAACATGGTTCAT1100.1975645676928051No Hit
GAGAAATATGGTGACCCATTTTCTTTGTGAAAACCTGGGAAAATGCAAGT1090.195768526168325No Hit
ACAATATACAATTTAAAAAAACTTATGGGGAAACACAGCTTATGTTTTTT1080.19397248464384495No Hit
TTTCTGTACATATCTTAAAAGGCAGAGATTACACTGATAAAGCCTAAAGA1070.1921764431193649No Hit
TAGTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTCTTTGTGAAAA1060.1903804015948849No Hit
GCTTAAGGGTTGCATACTTTTATTGGAGTAAATCTGAATGATCCTACTCC1060.1903804015948849No Hit
GTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGAAGACACAAAATTA1050.18858436007040483No Hit
CATTCAACCTGTTTATTACAACTTAAAAGGAACTTCAGTGAATTTGTTTT1040.1867883185459248No Hit
AAACAATACAGTACCATTTGAGTAACTGAGCAACATAATACCCATACTTT990.17780811092352455No Hit
TTCCATATCCTTTAATGAATTGCAAATATATTAACAGAAAAGACTTTCCC980.17601206939904449No Hit
AATATAAACAATACAGTACCATTTGAGTAACTGAGCAACATAATACCCAT960.17241998635008443No Hit
CTTCATACCCACACTTGCATTTTCCCAGGTTTTCACAAAGAAAATGGGTC950.17062394482560436No Hit
GTATTGTATTTGTGCAGGATTCTTTAGGCTTTATCAGTGTAATCTCTGCC940.16882790330112432No Hit
CCCATTTTCTTTGTGAAAACCTGGGAAAATGCAAGTGTGGGTATGAAGTG910.1634397787276842No Hit
GTACAGAAAATGTCCATATAAATTTCCATTGAAGTCGAATGATACTGAGA900.16164373720320413No Hit
GTTAATAACATTCAACCTGTTTATTACAACTTAAAAGGAACTTCAGTGAA890.1598476956787241No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.156255612629764No Hit
ATACAGTACCATTTGAGTAACTGAGCAACATAATACCCATACTTTATAGA870.156255612629764No Hit
GTATATAATGTTTAATATTTGTCAGAGCATTCTCCAGGTTTGCAGTTTTA860.15445957110528397No Hit
CTACAAATACAATATACAATTTAAAAAAACTTATGGGGAAACACAGCTTA850.1526635295808039No Hit
CTTGAGAGGTAATTTAAGTTTAAAGCAATTGACATTTTGAAGCATCTCCT820.14727540500736377No Hit
GGGAAACACAGCTTATGTTTTTTTCTCCTCTTTACAGGCTTCTCAGTATC820.14727540500736377No Hit
TTCTCCAGGTTTGCAGTTTTATTTCTATAAAGTATGGGTATTATGTTGCT810.14547936348288373No Hit
ATGTTATTTGGGGTACAAATATAAACAATACAGTACCATTTGAGTAACTG800.14368332195840366No Hit
GTACAGGGCCCAGCAGCATCTCAGCCTCCTACCCACAATTCCACTGAACA800.14368332195840366No Hit
GTACATGGGGATGATGCGGTGTGCCAGATTCGTCCCCCGCTTCTGCGCCG800.14368332195840366No Hit
GATAAAGCCTAAAGAATCCTGCACAAATACAATACAGAAAACAGAAAGTA800.14368332195840366No Hit
GAATAGGGTGTTTGATATTTCTTCATATGTTAAGGAGATGCTTCAAAATG790.14188728043392362No Hit
AAATTACATTGTTTCAATGCAAACAGAACACACACTTCATACCCACACTT790.14188728043392362No Hit
CCCCTCAACACTGTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGAA790.14188728043392362No Hit
GTATTTGTGCAGGATTCTTTAGGCTTTATCAGTGTAATCTCTGCCTTTTA780.14009123890944358No Hit
AGATTACACTGATAAAGCCTAAAGAATCCTGCACAAATACAATACAGAAA770.13829519738496354No Hit
CCCCCTCAACACTGTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGA760.1364991558604835No Hit
GTGTGTGGGTGGCCCCTTAACAGCCTAGAACTTTGGTGCACGTGCCCTCT750.13470311433600343No Hit
GTACAGACGATGTTATTTGGGGTACAAATATAAACAATACAGTACCATTT750.13470311433600343No Hit
CCTGAATAGTCAGGGAACTTTCACCTATTTTATTTAGGTTTTCTTTTTCT750.13470311433600343No Hit
GTACATGGGAAGGCGAGGACGGTGTGTGGGTGGCCCCTTAACAGCCTAGA750.13470311433600343No Hit
GTTTAATATTTGTCAGAGCATTCTCCAGGTTTGCAGTTTTATTTCTATAA740.1329070728115234No Hit
CGTCTGTACTTTCTGTTTTCTGTATTGTATTTGTGCAGGATTCTTTAGGC740.1329070728115234No Hit
ATATTAAACATTATATACACAATGAGGTAAATGTACCTTGGTCTCTTGAG740.1329070728115234No Hit
ACTATACCCTTTGTGAATAAAGAGATAATTATGTACGTGTAGCTACCTAT740.1329070728115234No Hit
GTATCAACGCAGAGTACATGGGAACCCTGGGAAAATTTGCAGAATCTAAG740.1329070728115234No Hit
CAATGAGGTAAATGTACCTTGGTCTCTTGAGAGGTAATTTAAGTTTAAAG740.1329070728115234No Hit
GTTAAGGAGATGCTTCAAAATGTCAATTGCTTTAAACTTAAATTACCTCT710.12751894823808327No Hit
CAGAAAAGACTTTCCCCCTCAACACTGTACAATTTCTGAAATAAGTCAGT700.1257229067136032No Hit
GTATTCACCTGAATAGTCAGGGAACTTTCACCTATTTTATTTAGGTTTTC690.12392686518912317No Hit
GGTATCAACGCAGAGTATAGTATGAGTCTTCACTGAGAAATATGGTGACC690.12392686518912317No Hit
ATTATATACACAATGAGGTAAATGTACCTTGGTCTCTTGAGAGGTAATTT680.12213082366464313No Hit
GTATCATTCGACTTCAATGGAAATTTATATGGACATTTTCTGTACATATC680.12213082366464313No Hit
AAATAACATCGTCTGTACTTTCTGTTTTCTGTATTGTATTTGTGCAGGAT680.12213082366464313No Hit
CTGTACAATTTCTGAAATAAGTCAGTTAAAGCAAAAAGAAGACACAAAAT680.12213082366464313No Hit
GTACATGGGGAAGTTTAAGTTGTCTAGCTTCGGTTCACAGGGCTTTAAGA680.12213082366464313No Hit
ATACAATACAGAAAACAGAAAGTACAGACGATGTTATTTGGGGTACAAAT680.12213082366464313No Hit
ATGTAATTTTGTGTCTTCTTTTTGCTTTAACTGACTTATTTCAGAAATTG670.12033478214016308No Hit
GAGTACATGGGAACCCTGGGAAAATTTGCAGAATCTAAGTTGATTGTAAT660.11853874061568304No Hit
ATATAATGTTTAATATTTGTCAGAGCATTCTCCAGGTTTGCAGTTTTATT650.11674269909120298No Hit
GTCAATAGATACAGAGAAGCCAAACTGAACAGCCTCAATAAAATAAAATT650.11674269909120298No Hit
GCAGAGTATAGTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTCTT650.11674269909120298No Hit
ACGCAGAGTATAGTATGAGTCTTCACTGAGAAATATGGTGACCCATTTTC640.11494665756672294No Hit
TTTGTGAACTGAATATCATGAACCATGTTTTGATACCCCTTTTTCACGTT640.11494665756672294No Hit
GTATCAACGCAGAGTACATGGGAAATGTAAACTTCTAAAAATATGGTTAA640.11494665756672294No Hit
CACCTGAATAGTCAGGGAACTTTCACCTATTTTATTTAGGTTTTCTTTTT630.1131506160422429No Hit
ACATTATATACACAATGAGGTAAATGTACCTTGGTCTCTTGAGAGGTAAT630.1131506160422429No Hit
CTTAAAAGGAACTTCAGTGAATTTGTTTTTATTTTTTAACAAGATTTGTG630.1131506160422429No Hit
ACCTATAGTTGTTTCTTTCCAACTCAAACACCATAACTATGTGGAAAAAG630.1131506160422429No Hit
TATATACACAATGAGGTAAATGTACCTTGGTCTCTTGAGAGGTAATTTAA620.11135457451776284No Hit
GTACATATCTTAAAAGGCAGAGATTACACTGATAAAGCCTAAAGAATCCT610.1095585329932828No Hit
AATTTGGACTGAATCCTAAATTTTTTGTAGCTACAAAGCTCCAAAATAAT610.1095585329932828No Hit
GAAAAGACTTTCCCCCTCAACACTGTACAATTTCTGAAATAAGTCAGTTA610.1095585329932828No Hit
CTCCTTAACATATGAAGAAATATCAAACACCCTATTCCGTTGGCACAACG580.10417040841984267No Hit
ATATTTGTCAGAGCATTCTCCAGGTTTGCAGTTTTATTTCTATAAAGTAT570.10237436689536261No Hit
CTTTAATGAATTGCAAATATATTAACAGAAAAGACTTTCCCCCTCAACAC570.10237436689536261No Hit
GCACAAATACAATACAGAAAACAGAAAGTACAGACGATGTTATTTGGGGT570.10237436689536261No Hit
GTATGGGTATTATGTTGCTCAGTTACTCAAATGGTACTGTATTGTTTATA570.10237436689536261No Hit
GATAAGGCCACTTGGGTTGCAGGCACACTGTGGAGTGTCAGGGGACAGAG560.10057832537088257No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)