FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19194
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT376519.615504845264145No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT12476.496821923517766RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT8434.391997499218506RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG6123.1884964051266023RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3441.7922267375221421RNA PCR Primer, Index 31 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT2221.156611441075336No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG2021.0524122121496302RNA PCR Primer, Index 38 (95% over 24bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA1921.000312597686777RNA PCR Primer, Index 31 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1170.6095654892153798No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC1090.5678857976450975No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1020.5314160675211004RNA PCR Primer, Index 31 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC920.4793164530582474RNA PCR Primer, Index 38 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT920.4793164530582474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.401167031363968No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC640.333437532562259No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTT620.32301760966968845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.29696780243826193No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC450.2344482650828384No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCT430.22402834219026777RNA PCR Primer, Index 38 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC420.2188183807439825No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGT420.2188183807439825No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGT400.20839845785141192No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTT340.1771386891737001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.15108888194227363No Hit
ATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT260.13545899760341773RNA PCR Primer, Index 38 (96% over 27bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACG230.11982911326456185No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGCTACGC210.10940919037199125No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTTCT210.10940919037199125RNA PCR Primer, Index 38 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)