FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9152
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT253827.731643356643353No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT8809.615384615384617TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7167.823426573426573TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT5936.479458041958042RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT2823.0812937062937062TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2793.048513986013986RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT2172.3710664335664338No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1421.5515734265734267TruSeq Adapter, Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT921.0052447552447552No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC890.972465034965035Illumina Paired End PCR Primer 2 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC860.9396853146853146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.8850524475524476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC660.7211538461538461No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT650.7102272727272727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.7102272727272727No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT610.666520979020979No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT470.513548951048951No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT430.4698426573426573No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG420.4589160839160839Illumina PCR Primer Index 5 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT370.40428321678321677Illumina PCR Primer Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC350.38243006993006995No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT320.34965034965034963No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT300.3277972027972028TruSeq Adapter, Index 10 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.3277972027972028No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT280.30594405594405594No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA260.2840909090909091TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCT210.22945804195804195Illumina PCR Primer Index 5 (95% over 21bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC210.22945804195804195No Hit
CTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTGA190.2076048951048951Illumina PCR Primer Index 5 (96% over 26bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC190.2076048951048951No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCT160.17482517482517482Illumina PCR Primer Index 5 (95% over 24bp)
TCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTG160.17482517482517482Illumina PCR Primer Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGG140.15297202797202797No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTT140.15297202797202797No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTT140.15297202797202797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.15297202797202797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.14204545454545456No Hit
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGGGATCTCGGCTCAC120.13111888111888112No Hit
CTTATCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC120.13111888111888112No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCC120.13111888111888112No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGAGGCT110.1201923076923077No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTG110.1201923076923077Illumina PCR Primer Index 5 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.1201923076923077No Hit
TCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTTCTGCTTG100.10926573426573427Illumina PCR Primer Index 5 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10926573426573427Clontech SMART CDS Primer II A (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCCT100.10926573426573427No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAAATCGTATGCCGT100.10926573426573427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)