FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98159
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT1273812.976904817693741No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT72847.420613494432502TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT60236.135963080308479RNA PCR Primer, Index 3 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24272.472519076192708No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG14071.4333886857038072TruSeq Adapter, Index 3 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13271.351888262920364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11931.2153750547580966No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG8270.842510620523844No Hit
GTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8150.8302855571063275No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC7720.7864790798602268No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT6510.6632096904002689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.6326470318564777No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.4767774732831426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.42074593261952553No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG3490.35554559439277095TruSeq Adapter, Index 3 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3330.3392455098360823Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3040.3097016065770841Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT3000.30562658543791194TruSeq Adapter, Index 3 (96% over 26bp)
GTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT2810.2862702350268442RNA PCR Primer, Index 3 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.2781201927484999No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.22514491793926178No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC2130.21699487566091746No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1986572805346428Clontech SMART CDS Primer II A (100% over 21bp)
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1930.1966197699650567Illumina PCR Primer Index 12 (95% over 24bp)
GCACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT1490.15179453743416293TruSeq Adapter, Index 3 (95% over 23bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1480.1507757821493699RNA PCR Primer, Index 31 (96% over 29bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1470.14975702686457684Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1140.11613810246640655RNA PCR Primer, Index 33 (96% over 29bp)
GTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1110.11308183661202743No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG1080.11002557075764832RNA PCR Primer, Index 27 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1050.10696930490326918No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1050.10696930490326918Clontech SMART CDS Primer II A (96% over 26bp)
GACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1050.10696930490326918TruSeq Adapter, Index 3 (95% over 24bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1030.1049317943336831Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1030.1049317943336831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1020.10391303904889006TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)