FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130442
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT2594219.887766210269696No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT105148.060287330767697RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT59374.551448153202189RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44283.394612164793548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31582.4209993713681177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26492.030787629751154No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC16031.228898667607059No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG13881.0640744545468483TruSeq Adapter, Index 4 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11700.8969503687462627No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT11160.8555526594195121No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.8302540592753868No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.7811901074807194No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT7330.5619355728982995RNA PCR Primer, Index 46 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG7220.5535027061835912RNA PCR Primer, Index 46 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.5519694576900078Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.42777632970975604No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC5550.4254764569693811No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG4570.3503472807837966No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3790.2905505895340458No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.25911899541558703No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC3240.24838625596050354TruSeq Adapter, Index 18 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3030.2322871467778783No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2530.19395593443829443Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT2280.17479032826850247No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.16175771607304396No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2040.1563913463455022Clontech Universal Primer Mix Long (96% over 26bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1970.15102497661796047RNA PCR Primer, Index 46 (96% over 28bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.1464252311372104No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT1860.14259210990325202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGT1750.13415924318854355No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT1720.13185937044816853No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.11652688551233499No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGATCTCGTATGCCGTC1460.11192714003158492RNA PCR Primer, Index 33 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1370.10502752181045982Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)