FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275983
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT3224111.682241297471222No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT122484.437954511690938RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88253.1976607254794684No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT68722.4900084425489974RNA PCR Primer, Index 31 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62972.281662276299627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54431.972222926774475No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.8775902863582177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.8304859357279253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.8272248652996743No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC16820.6094578289242454No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG13380.4848124703333176RNA PCR Primer, Index 38 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT13030.4721305297790081RNA PCR Primer, Index 31 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.3942271806596783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.39277781602490003Clontech SMART CDS Primer II A (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.2956703854947587No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC8100.2934963385425914No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC5450.1974759314885337No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC4280.15508201592127052No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC4080.14783519274737936No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.1442117811604338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3900.14131305189087734Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCA3560.1289934524952624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3490.1264570643844005Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.12029726468659302No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.11594917078225832No Hit
CTCTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGTTGCG3200.11594917078225832No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG3120.11305044151270188No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG2790.10109318327578148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)