FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275983
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83923.0407670037647248No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28361.0275995260577644No Hit
GTACATGGGGAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCA4000.1449364634778229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.1322545229235134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3590.13008047597134606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.12609472322570595No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC3330.12065960584528757No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3060.11087639456053454Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGTTGCG2890.10471659486272705No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT2770.10036850095839238No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)