FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001421905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279420.69855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167310.418275No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT128020.32005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116080.2902No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT88100.22025No Hit
TCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATAATGGAA55920.13979999999999998No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG54640.1366No Hit
CAATAACACGGCACATAAAAAAGATTAAATTAAGAGAAGGGACAGGAACT51370.128425No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT50140.12535000000000002TruSeq Adapter, Index 3 (95% over 21bp)
GTCCAGGAGTACGTGTATGACCTGGAACTGAACTGAGCTGCTCAGAGACA44500.11125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)