FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001421907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477503
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT179020.5147946673230763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124520.3580730196350657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100800.2898631575587426No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT71300.20503217394780104RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70930.20396819211946043No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT42180.12129392843083096RNA PCR Primer, Index 46 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)