FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG963.662724151087371TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG923.510110644792064RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG803.052270125906143RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG712.708889736741702RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG672.556276230446395RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA411.564288439526898RNA PCR Primer, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG401.5261350629530714RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG391.4879816863792446RNA PCR Primer, Index 39 (96% over 30bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA341.2972148035101108RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA321.2209080503624572RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT291.1064479206409767RNA PCR Primer, Index 46 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT271.0301411674933232RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG271.0301411674933232TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA260.9919877909194963RNA PCR Primer, Index 31 (96% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA260.9919877909194963RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG240.9156810377718427RNA PCR Primer, Index 23 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT230.877527661198016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.8393742846241892No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG190.7249141549027089TruSeq Adapter, Index 4 (95% over 23bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA190.7249141549027089RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT170.6486074017550554TruSeq Adapter, Index 1 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.6486074017550554No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT150.5723006486074018RNA PCR Primer, Index 33 (96% over 27bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT140.534147272033575RNA PCR Primer, Index 46 (96% over 26bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA130.49599389545974815RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT130.49599389545974815RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT130.49599389545974815RNA PCR Primer, Index 31 (96% over 27bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT130.49599389545974815RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA120.45784051888592137RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT110.4196871423120946RNA PCR Primer, Index 17 (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.4196871423120946No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT100.38153376573826786RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA90.3433803891644411RNA PCR Primer, Index 10 (96% over 28bp)
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA80.3052270125906143TruSeq Adapter, Index 1 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3052270125906143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.2670736360167875No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT70.2670736360167875TruSeq Adapter, Index 1 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.2670736360167875No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA60.22892025944296068TruSeq Adapter, Index 10 (96% over 28bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA60.22892025944296068RNA PCR Primer, Index 26 (96% over 31bp)
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA60.22892025944296068RNA PCR Primer, Index 38 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT50.19076688286913393TruSeq Adapter, Index 1 (95% over 21bp)
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC50.19076688286913393Illumina PCR Primer Index 5 (95% over 24bp)
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT50.19076688286913393RNA PCR Primer, Index 27 (100% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT50.19076688286913393RNA PCR Primer, Index 31 (95% over 24bp)
GAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAA50.19076688286913393TruSeq Adapter, Index 25 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT50.19076688286913393RNA PCR Primer, Index 27 (96% over 25bp)
TCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA50.19076688286913393TruSeq Adapter, Index 12 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC50.19076688286913393No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG50.19076688286913393RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT40.15261350629530715RNA PCR Primer, Index 33 (95% over 24bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC40.15261350629530715RNA PCR Primer, Index 15 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG40.15261350629530715RNA PCR Primer, Index 27 (100% over 24bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG40.15261350629530715RNA PCR Primer, Index 15 (95% over 24bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.15261350629530715No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG40.15261350629530715TruSeq Adapter, Index 1 (95% over 24bp)
GTCCAGAGGCTTTAAAACTGGTGCAATTACAGCAAAAAGGGATTCTGTAG40.15261350629530715No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT40.15261350629530715RNA PCR Primer, Index 26 (96% over 30bp)
GAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA40.15261350629530715Illumina PCR Primer Index 1 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15261350629530715No Hit
TCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGTCTTCTGCTTG40.15261350629530715TruSeq Adapter, Index 5 (96% over 26bp)
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA40.15261350629530715RNA PCR Primer, Index 27 (100% over 28bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT40.15261350629530715No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC40.15261350629530715RNA PCR Primer, Index 38 (96% over 25bp)
GAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA40.15261350629530715RNA PCR Primer, Index 38 (96% over 28bp)
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA30.11446012972148034Illumina PCR Primer Index 1 (96% over 27bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA30.11446012972148034RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT30.11446012972148034TruSeq Adapter, Index 10 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG30.11446012972148034No Hit
GGGGAGACAATGCTATCTTGTTAGGACATAGCTTTCCATGATGTCTAGGA30.11446012972148034No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG30.11446012972148034Illumina Single End Adapter 1 (100% over 25bp)
TCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTTCTGCTTG30.11446012972148034Illumina PCR Primer Index 5 (96% over 26bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC30.11446012972148034TruSeq Adapter, Index 27 (96% over 25bp)
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA30.11446012972148034Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30.11446012972148034No Hit
CTCTAGAGTTGTGTGCGCGTTTTAAGGAAAACTGAGGGGTAGGTAAAGAA30.11446012972148034No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG30.11446012972148034TruSeq Adapter, Index 11 (96% over 28bp)
GAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA30.11446012972148034Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCTGCTTGA30.11446012972148034RNA PCR Primer, Index 26 (96% over 29bp)
TTCTAATTCTAGTCCATGTTTCAACTTCTTTTGTACTTTTCATCTCTTAT30.11446012972148034No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTAGGCAT30.11446012972148034No Hit

[OK]Adapter Content

Adapter graph