FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461.755055322396032No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT140.534147272033575Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG80.3052270125906143No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC80.3052270125906143No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT30.11446012972148034Illumina RNA PCR Primer (100% over 25bp)
ATACTGCTCTGTCCAGAGGCTTTAAAACTGGTGCAATTACAGCAAAAAGG30.11446012972148034No Hit
GCCTAAGAGTTATAGGCTTAAAGATGTCGAGTAACTAAAAACTGTATTGC30.11446012972148034No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT30.11446012972148034Illumina RNA PCR Primer (96% over 25bp)
CTCTATATACAGGACGGACAGCAAAATTCTCACCTTCCCAAAGGCATGTG30.11446012972148034No Hit
GAATTTACACTCTGTTTAAATGATGGGATTCTATCGAGATAGCACTCATG30.11446012972148034No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11446012972148034No Hit
ATTTGGGACCAGACAAAATCAGCTTTCATCTATAATTCATTGGGGATAAT30.11446012972148034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)