FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282537
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.340840314719842No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT6430.2275808124245674TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.2197942216417673No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT5830.2063446557442034RNA PCR Primer, Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.1957265774040214No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT4520.15997904699207538No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG4100.14511373731582058RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG3260.115383117963311RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT3130.11078195068256547TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG2910.10299535989976534TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG2880.10193355206574714RNA PCR Primer, Index 31 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)