FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409683
Sequences flagged as poor quality0
Sequence length100
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT5514013.45918673706256No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT234085.713685947427645TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220125.3729346836456475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191964.685573968165631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134063.272286133425112No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT134023.271309768772441RNA PCR Primer, Index 1 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92902.2676069058271886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70971.732314985000598No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65151.5902539280370431No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG29990.7320293983396919TruSeq Adapter, Index 1 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.7027384587595775No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.6744238838321337No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC26130.6378102093569906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.6256056511986097Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT21990.536756467805596No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG17430.42545089740116143TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT17080.4169077066902947TruSeq Adapter, Index 1 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC12250.2990116748803343No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.24433525433078745No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC9110.22236704964570167No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8710.21260340311899686No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.21162703846632638No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT8590.20967430916098545No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8060.19673747751310158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7490.18282428121254726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7410.1808715519072063Clontech Universal Primer Mix Long (96% over 26bp)
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC6860.16744653793298722Illumina PCR Primer Index 5 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6470.15792698256945004No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT5410.13205331927368233Clontech SMART CDS Primer II A (95% over 21bp)
ATATAATAGGATACTATGTGGTCACAAAAAATCAGGCTATGGAAAAGTAT5230.12765967833666517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5200.1269274048471623No Hit
TCCTTAGAGTGTCATGTGACACTCAAATAGTTTCAGATTTTGGAGCATTT4930.12033694344163659No Hit
GTATATGGGCGGAGGCGCAGGAACTCCTTGTGTTGCCTGGAGACGAAGTT4800.11716375832045753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.1144787555256137No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG4680.11423466436244609No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4680.11423466436244609Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTG4670.11399057319927847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4660.11374648203611085No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA4640.11325829970977562RNA PCR Primer, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT4590.1120378438939375No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4530.1105732969149318No Hit
ATATTAAGGAGTTTGTAGGTGAAGTTTAATCTGTGCATAGTGGGTAGAGA4450.10862056760959082No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4410.10764420295692036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAGAGTGGTCACCTCTCAGTTTCACTATGAGCACAATTTAAATGT4400.10740011179375274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4390.10715602063058512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4310.10520329132524416No Hit
CTCTTAATATTTTGTGGCATAAGCATTCCAGTCTTTTATCTTTCTCAAAG4280.1044710178357413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4200.10251828853040035No Hit
GTTTAAACTGTGTACATTCTCCACAGATCATATTAAGGAGTTTGTAGGTG4150.10129783271456223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)