FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409683
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA308637.5333855688422515No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49851.216794448390585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11080.2704530087897228No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT10990.26825618832121423Illumina RNA PCR Primer (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC9330.2277370552353893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC8730.21309158544533213No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8710.21260340311899686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC8670.21162703846632638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC8300.20259566542912447No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG7680.18746201331273205No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7550.18428882819155298Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC7050.172084270033172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.15914743838528814No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.14206105696355473No Hit
ATATAATAGGATACTATGTGGTCACAAAAAATCAGGCTATGGAAAAGTAT5390.1315651369473471No Hit
GTATCAACGCAGAGTACTTCTGCTTTTTCTTTCTTTTTATTTTAAATCTG4940.12058103460480421No Hit
GTATATGGGCGGAGGCGCAGGAACTCCTTGTGTTGCCTGGAGACGAAGTT4910.11984876111530135No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4800.11716375832045753No Hit
ATATTAAGAGTGGTCACCTCTCAGTTTCACTATGAGCACAATTTAAATGT4530.1105732969149318No Hit
AAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.10788829412008798No Hit
CTCTTAATATTTTGTGGCATAAGCATTCCAGTCTTTTATCTTTCTCAAAG4320.10544738248841179No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC4250.10373874434623843No Hit
GTACATGGGCAATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCC4210.10276237969356797No Hit
TCCTTAGAGTGTCATGTGACACTCAAATAGTTTCAGATTTTGGAGCATTT4200.10251828853040035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCC4190.10227419736723271No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4190.10227419736723271No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)