FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202246
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT554924.6156942921831305No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT272882.269751781249428RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191251.5907726039429533No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT187911.5629912680100413RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145951.2139778381462696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118070.9820785429936968No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG69700.5797482378814319RNA PCR Primer, Index 23 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65240.5426510048692198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.48542477995352035No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT50980.42403967241313345RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG50290.4183004143910647Illumina PCR Primer Index 1 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.39135085498309No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC28660.23838715204708522No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT25510.2121861915115542RNA PCR Primer, Index 23 (95% over 22bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA23160.19263944317552315RNA PCR Primer, Index 23 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.1743403596268983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.17342540544946708Clontech SMART CDS Primer II A (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.16860110160482963No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC18630.15495996659585476Illumina PCR Primer Index 1 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)