FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7924
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGCCCAGGCAGGTCTCGAACTCCTGGGCTCAAGCTATCCTCCCGCC140.17667844522968199No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT130.1640585562847047No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT120.1514386673397274Illumina Single End PCR Primer 1 (100% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12619888944977284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90.11357900050479555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG80.10095911155981827No Hit
GAACGGGGTCTCGCTATATTGCCCAGGCAGGTCTCGAACTCCTGGGCTCA80.10095911155981827No Hit
CTCCTGGGCTCAAGCTATCCTCCCGCCTCTTAGCCTCCCTGAGAGCTGGG80.10095911155981827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)