FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8584
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT243528.36672879776328No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT85910.00698974836906RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT6117.117893755824791RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG5736.675209692451071RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT2713.1570363466915192RNA PCR Primer, Index 33 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT2302.679403541472507No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1772.061975768872321RNA PCR Primer, Index 33 (96% over 29bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG1331.549394221808015RNA PCR Primer, Index 15 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT961.1183597390493942No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC850.9902143522833178RNA PCR Primer, Index 15 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT850.9902143522833178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.9902143522833178No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC830.9669151910531221No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC750.8737185461323392No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT650.7572227399813607RNA PCR Primer, Index 33 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.7572227399813607No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCT610.7106244175209693Illumina PCR Primer Index 4 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.4776328052190121No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT400.4659832246039142No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC380.4426840633737186No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT380.4426840633737186No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT320.3727865796831314RNA PCR Primer, Index 15 (96% over 27bp)
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC320.3727865796831314No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC300.3494874184529357No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG290.33783783783783783Illumina PCR Primer Index 4 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTT230.2679403541472507No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.25629077353215285No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCT200.2329916123019571Illumina PCR Primer Index 4 (95% over 24bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC190.2213420316868593No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.19804287045666358Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCTGCTTG160.1863932898415657Illumina PCR Primer Index 4 (96% over 26bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG160.1863932898415657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC160.1863932898415657No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCT150.17474370922646784Illumina PCR Primer Index 4 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC150.17474370922646784No Hit
CTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTGA150.17474370922646784Illumina PCR Primer Index 4 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT140.16309412861137No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAGGCAATCTCGTATGCCGT140.16309412861137No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCATCTCGTATGCCGTC130.15144454799627213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC130.15144454799627213No Hit
CTTATCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC110.12814538676607642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC110.12814538676607642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.12814538676607642No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAAATCGTATGCCGT100.11649580615097856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCC100.11649580615097856No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTC90.1048462255358807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC90.1048462255358807No Hit
TCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA90.1048462255358807RNA PCR Primer, Index 15 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTT90.1048462255358807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)