FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT219926.72906284186216No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT85810.429074996961225TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT5997.280904339370366RNA PCR Primer, Index 3 (95% over 23bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG5857.110732952473563TruSeq Adapter, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT2573.123860459462745TruSeq Adapter, Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT2262.747052388476966No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1932.345934119363073RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1591.932660751185122TruSeq Adapter, Index 3 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT931.1304242129573356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT831.008873222316762No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT780.948097726996475No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC730.8873222316761883No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC660.8022365382277865Illumina PCR Primer Index 12 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT660.8022365382277865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC610.7414610429074997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.7414610429074997No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT540.6563753494590981Illumina PCR Primer Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC390.4740488634982375No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG380.4618937644341801Illumina PCR Primer Index 8 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT350.425428467242008No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG310.37680807098577856No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC300.3646529719217212No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT290.3524978728576638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.31603257566549164No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT250.30387747660143427TruSeq Adapter, Index 3 (96% over 27bp)
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT240.2917223775373769Illumina PCR Primer Index 8 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT230.2795672784733195Illumina PCR Primer Index 8 (95% over 21bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG190.23094688221709006No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTC190.23094688221709006No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG180.2187917831530327Illumina PCR Primer Index 8 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT170.20663668408897531No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT170.20663668408897531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC160.19448158502491797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.17017138689680322Clontech SMART CDS Primer II A (100% over 21bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG140.17017138689680322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.17017138689680322No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA130.15801628783274582TruSeq Adapter, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG130.15801628783274582Illumina PCR Primer Index 8 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT120.14586118876868845No Hit
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA120.14586118876868845Illumina PCR Primer Index 8 (96% over 26bp)
CTTATAACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG120.14586118876868845No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGATCTCGTATGCCGTC100.12155099064057372No Hit
CTTATCACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC100.12155099064057372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.12155099064057372No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCCT90.10939589157651634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC90.10939589157651634No Hit
CTCGGAGATGTGTATAAGAGACAGATCTCCGAGCCCACGAGACGTAGAGG90.10939589157651634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)