FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT236031.614199598124582No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT76410.234427327528465RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5136.872069658405894RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG4686.269256530475554RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1972.6389819156061622RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA1401.8754186202277294RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT1371.8352310783657066No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1151.5405224380442064RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC791.058271935699933RNA PCR Primer, Index 26 (95% over 22bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC730.9778968519758876RNA PCR Primer, Index 26 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT730.9778968519758876RNA PCR Primer, Index 26 (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC690.9243134628265238No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT680.9109176155391828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.696584058941728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.696584058941728No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT430.5760214333556598RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT370.49564634963161425No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC320.4286671131949095No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC290.3884795713328868RNA PCR Primer, Index 26 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT290.3884795713328868RNA PCR Primer, Index 26 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.348292029470864No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT230.30810448760884124RNA PCR Primer, Index 26 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG200.26791694574681846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.24112525117213662No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG160.21433355659745476RNA PCR Primer, Index 26 (96% over 29bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT150.20093770931011384RNA PCR Primer, Index 26 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.20093770931011384No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT140.18754186202277295No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT130.174146014735432RNA PCR Primer, Index 26 (95% over 24bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA130.174146014735432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC120.1607501674480911No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1607501674480911No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCTTG110.14735432016075017RNA PCR Primer, Index 26 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.14735432016075017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.13395847287340923Clontech SMART CDS Primer II A (100% over 21bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA100.13395847287340923RNA PCR Primer, Index 26 (96% over 31bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG90.12056262558606831TruSeq Adapter, Index 1 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12056262558606831No Hit
CTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTGA90.12056262558606831RNA PCR Primer, Index 26 (96% over 29bp)
ACTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG80.10716677829872738No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG80.10716677829872738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC80.10716677829872738No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG80.10716677829872738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC80.10716677829872738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG80.10716677829872738No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCC80.10716677829872738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)