FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11172
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT339830.415324024346578No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10529.41639813820265RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT7196.435732187611887RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG6095.451127819548872RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT2812.515216612960974RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA1941.736484067311135RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT1781.593268886501969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1671.4948084496956677No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG1561.3963480128893664RNA PCR Primer, Index 27 (100% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1291.154672395273899No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1080.966702470461869No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1030.9219477264590046No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC900.8055853920515575TruSeq Adapter, Index 27 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT870.7787325456498388RNA PCR Primer, Index 27 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT850.7608306480486932No Hit
ACGCAGAGCAGTCTGTTTAGCTGTGAACACCACAATCTGCAGATGTTCAA830.7429287504475474No Hit
TATAAATACTATGCCATTTTATGTAAGGGACTTGAACATCTGCAGATTGT810.7250268528464017No Hit
GTATCAACGCAGAGCAGTCTGTTTAGCTGTGAACACCACAATCTGCAGAT760.6802721088435374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.6086645184389545No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT640.5728607232366631No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCT550.49230218403150733RNA PCR Primer, Index 38 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC490.43859649122807015RNA PCR Primer, Index 27 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC460.4117436448263516No Hit
GTTTAGCTGTGAACACCACAATCTGCAGATGTTCAAGTCCCTTACATAAA440.39384174722520593No Hit
GGTATCAACGCAGAGCAGTCTGTTTAGCTGTGAACACCACAATCTGCAGA430.38489079842463303No Hit
TATCAACGCAGAGCAGTCTGTTTAGCTGTGAACACCACAATCTGCAGATG410.3669889008234873No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.322234156820623Clontech SMART CDS Primer II A (100% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT350.3132832080200501No Hit
CTGTTTAGCTGTGAACACCACAATCTGCAGATGTTCAAGTCCCTTACATA300.26852846401718583No Hit
GGTTACATATAAATACTATGCCATTTTATGTAAGGGACTTGAACATCTGC280.2506265664160401No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT260.2327246688148944No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTT260.2327246688148944No Hit
AAAATATACAGGAGAATATGCATAGGTTACATATAAATACTATGCCATTT230.2058718224131758No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC230.2058718224131758No Hit
CATATAAATACTATGCCATTTTATGTAAGGGACTTGAACATCTGCAGATT220.19692087361260296No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTC200.17901897601145722No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCT200.17901897601145722RNA PCR Primer, Index 38 (95% over 21bp)
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT190.17006802721088435RNA PCR Primer, Index 27 (100% over 27bp)
GGAGAATATGCATAGGTTACATATAAATACTATGCCATTTTATGTAAGGG180.1611170784103115No Hit
GTAGAGATGATTTAAAATATACAGGAGAATATGCATAGGTTACATATAAA170.15216612960973863No Hit
ATGTAGAGATGATTTAAAATATACAGGAGAATATGCATAGGTTACATATA160.1432151808091658No Hit
ATGCATAGGTTACATATAAATACTATGCCATTTTATGTAAGGGACTTGAA160.1432151808091658No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGGACTCC150.13426423200859292No Hit
GAGCAGTCTGTTTAGCTGTGAACACCACAATCTGCAGATGTTCAAGTCCC150.13426423200859292No Hit
ATACAGGAGAATATGCATAGGTTACATATAAATACTATGCCATTTTATGT150.13426423200859292No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTT150.13426423200859292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13426423200859292No Hit
TCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCTTG150.13426423200859292RNA PCR Primer, Index 38 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCC130.1163623344074472No Hit
ATATACAGGAGAATATGCATAGGTTACATATAAATACTATGCCATTTTAT130.1163623344074472No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCCTCT130.1163623344074472RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC130.1163623344074472No Hit
GAATATGCATAGGTTACATATAAATACTATGCCATTTTATGTAAGGGACT130.1163623344074472No Hit
GTATTTTAATGTAGAGATGATTTAAAATATACAGGAGAATATGCATAGGT120.10741138560687433No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10741138560687433No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)