FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7083
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT182725.794155019059723No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT5998.456868558520402TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4235.972045743329097RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG4225.957927431879147TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT1682.3718763235916986No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA1582.2306932090921925RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT1572.216574897642242TruSeq Adapter, Index 10 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG801.1294649159960468TruSeq Adapter, Index 10 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT751.058873358746294No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT711.0024001129464915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC690.9741634900465904No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT560.7906254411972328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.7623888182973316No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC530.7482705068473811No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC520.7341521953974305Illumina Paired End PCR Primer 2 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT520.7341521953974305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.578850769447974No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT400.5647324579980234No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT350.49414090074827055Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT320.4517859663984188No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC290.40943103204856696No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG220.3106028518989129Illumina PCR Primer Index 5 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTG210.29648454044896233Illumina PCR Primer Index 5 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.2823662289990117Clontech SMART CDS Primer II A (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT200.2823662289990117No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC180.25412960609911056No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTT160.2258929831992094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.2258929831992094No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT160.2258929831992094No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCT140.1976563602993082Illumina PCR Primer Index 5 (95% over 24bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT130.18353804884935762TruSeq Adapter, Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAAATCGTATGCCGT120.16941973739940702No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCT110.15530142594945645Illumina PCR Primer Index 5 (95% over 21bp)
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA110.15530142594945645TruSeq Adapter, Index 10 (96% over 28bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC110.15530142594945645No Hit
GTACATGGGAGAGTTATCTGGGATTCTGATTTTTTAAATAGTACATATCA100.14118311449950585No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.14118311449950585No Hit
GTATCAACGCAGAGTACATGGGAGAGTTATCTGGGATTCTGATTTTTTAA90.12706480304955528No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG90.12706480304955528No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTT80.1129464915996047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC80.1129464915996047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)