FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86454
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20922.419783931339209No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9161.0595229833206097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.8571031993892706No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT6390.73912138246929RNA PCR Primer, Index 33 (95% over 22bp)
ACATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAGTT5540.6408032017026395No Hit
ACATAACACATTGCATGCTACATAATACACTGTAACCTTCTAGCAGGGGT5240.60610266731441No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT4870.5633053415689269RNA PCR Primer, Index 33 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.4915909038332523No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA3910.4522636315265922No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT3680.42565988849561615No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG3680.42565988849561615RNA PCR Primer, Index 33 (96% over 29bp)
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT3630.4198764660975779No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT3500.4048395678626784No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC3230.3736090869132718No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA3050.35278876628033407No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2990.3458486594026881No Hit
GAATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGC2940.34006523700464986No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT2890.3342818146066116No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT2690.3111481250144586No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA2430.2810743285446596No Hit
GTACATGGGAGGAGGCCAAGCGGCAGAGATTGCCAATGCCAAGTCCAGAG2420.27991764406505193No Hit
GAAGTAGGGTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATTC2410.2787609595854443No Hit
ATTTAATAGTTCAGACATCTTAGACAAGACTTGACTTTTGGGCTTCAGCA2370.2741342216670137No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG2310.2671941147893678No Hit
GTATAACCCACTGCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTCT2220.25678395447289887No Hit
CTACATAACACATTGCATGCTACATAATACACTGTAACCTTCTAGCAGGG2130.24637379415643001No Hit
GGATTATGATGCGACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAG2060.23827700279917646No Hit
TCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTT1970.22786684248270755No Hit
TGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCCTT1950.22555347352349225No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC1910.22092673560506165No Hit
GAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGCACAGAGA1890.21861336664584632No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT1890.21861336664584632No Hit
ATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAGTTAT1880.21745668216623867No Hit
TCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGGAAACTTTTT1850.21398662872741575No Hit
GGCCATAACTGAGTTATTTTTTTCATCAATCAGGAAAATGCTTCCTTAAT1830.21167325976820042No Hit
GGGTTATACCTTGGCACCTAAATAAAAGATACAATAGGAAACGATTCTTT1830.21167325976820042No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT1820.21051657528859277No Hit
TTCCAGGACTGGCTACTGCGTACATCCACAGGTTCACTAACGGGGGCAAG1800.2082032063293775No Hit
CCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTACTTGCCG1780.20588983737016217No Hit
TCTTCAGACTGGTCAAGAAAGAGCTAGACCTGGTGCTTGGAGAAGAGACA1760.20357646841094684No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT1760.20357646841094684RNA PCR Primer, Index 33 (96% over 27bp)
AGATAAAGTTGTCCAGGAAAGAGAAGATGCCCTAAGGCTTCTTCAGACTG1680.19432299257408564No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT1630.18853957017604736No Hit
GGTATCAACGCAGAAGTAGGGTTTTGGCCTAGGTAACGGGGCAGAGATGT1560.1804427788187938No Hit
GCCTTATGTGGACCATTTTCTCAGACTGGAACGTGAGAAACGAGCCCGCA1550.17928609433918616No Hit
GGATAGTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAA1520.17581604090036318No Hit
TCCTTAATCAATGTTCTCCAAACCCTTTGACACATAGTAACGATCAACTC1500.17350267194114788No Hit
TCTCTGGAGTTGATCGTTACTATGTGTCAAAGGGTTTGGAGAACATTGAT1470.17003261850232493No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT1390.1607791426654637No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.15962245818585608No Hit
GTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA1380.15962245818585608No Hit
GGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCATGGGCGTG1380.15962245818585608No Hit
ACATAAGGCAAGGCAAAGAATCGTTTCCTATTGTATCTTTTATTTAGGTG1360.15730908922664075No Hit
GTATCAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTT1350.1561524047470331No Hit
GACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGC1340.15499572026742545No Hit
GGTATCAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCT1320.15268235130821015No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGA1320.15268235130821015No Hit
GAAAAAAGGGTTGCTCATTTTGGGTATCACTGGAGTCTGATGGAAAGAGA1300.15036898234899485No Hit
AGGTAACGGGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCA1300.15036898234899485No Hit
CTACATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAG1300.15036898234899485No Hit
TTCCTACACTTTGCCTAATTGAGCCACTACCAGGTTTTCTGGCAGCTGTG1290.1492122978693872No Hit
GTTCAGACATCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGG1280.14805561338977952No Hit
AATTTACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCACTC1270.1468989289101719No Hit
TTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTGAGCCAAC1260.14574224443056422No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.14458555995095657No Hit
CCCCTACTCATCGCACTAATTTACACTCACAACACCCTAGGCTCACTAAA1230.14227219099174127No Hit
GGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGTGTGAGGCGTA1230.14227219099174127No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1220.14111550651213361No Hit
GCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTCTTCAATCAGC1210.13995882203252596No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT1200.13880213755291831No Hit
TCACTGGAGTCTGATGGAAAGAGATAGGCGCATCTCTGGAGTTGATCGTT1190.13764545307331066No Hit
ATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACA1180.136488768593703No Hit
TACTAGCACAGAGAGTTCTCCCAGTAGGTTAATAGTGGGGGGTAAGGCGA1180.136488768593703No Hit
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA1180.136488768593703No Hit
GTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCGGTG1170.13533208411409536No Hit
ATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG1150.13301871515488004No Hit
ACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTT1150.13301871515488004No Hit
GCCCAAGAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTA1150.13301871515488004No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1140.1318620306752724RNA PCR Primer, Index 33 (96% over 29bp)
GGTATAACCCACTGCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTC1130.13070534619566476No Hit
GGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCAT1110.12839197723644943No Hit
CTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAA1100.12723529275684178No Hit
GTACATGGGAACCAAAATCGATATCAGCAATGTAAAAATCCCAAAACATC1100.12723529275684178No Hit
GGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTG1090.12607860827723413No Hit
AGTATGTTGAGTCCTGTGAGTAGGAGAGTGATATTTGATCAGGAGAACGT1080.12492192379762648No Hit
GTATCAACGCAGAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGT1070.12376523931801883No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG1070.12376523931801883RNA PCR Primer, Index 15 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.12376523931801883No Hit
ATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCAT1050.12145187035880352No Hit
CTCCAGAGATGCGCCTATCTCTTTCCATCAGACTCCAGTGATACCCAAAA1050.12145187035880352No Hit
ATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCG1040.12029518587919588No Hit
CCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGT1030.11913850139958823No Hit
CTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAACAC1030.11913850139958823No Hit
CTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCAC1020.11798181691998057No Hit
CCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTA1010.1168251324403729No Hit
TATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTA1010.1168251324403729No Hit
GCGTACATCCACAGGTTCACTAACGGGGGCAAGGAAAAAAGGGTTGCTCA1010.1168251324403729No Hit
GCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGC1000.11566844796076527No Hit
CCTCTTTACGGACTCCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCC1000.11566844796076527No Hit
CTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTC990.11451176348115762No Hit
TTGCATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTC990.11451176348115762No Hit
CCCTAAGGCTTCTTCAGACTGGTCAAGAAAGAGCTAGACCTGGTGCTTGG970.11219839452194232No Hit
GTATCAACGCAGAGTACATGGGAACCAAAATCGATATCAGCAATGTAAAA970.11219839452194232No Hit
CTCCTACTCACAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTA970.11219839452194232No Hit
ATCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGGAAACTTTT960.11104171004233465No Hit
CATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTC950.109885025562727No Hit
GCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTC950.109885025562727No Hit
GGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTC950.109885025562727No Hit
GGTCAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATA950.109885025562727No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.109885025562727Clontech SMART CDS Primer II A (100% over 21bp)
CACAACACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCA950.109885025562727No Hit
CTATTGACCCAGCGATGGGGGCTTCGACATGGGCTTTAGGGAGTCATAAG940.10872834108311934No Hit
GTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGATGGAGCTTG940.10872834108311934No Hit
ACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATA940.10872834108311934No Hit
CTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTCTA930.1075716566035117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.10641497212390404No Hit
CAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATA910.10525828764429639No Hit
GTCATAAGTGGAGTCCGTAAAGAGGTATCTTTACTATAAAAGCTATTGTG900.10410160316468875No Hit
GTCCTTGAGAGAGGATTATGATGCGACTGTGAGTGCGTTCGTAGTTTGAG900.10410160316468875No Hit
CACTAATAGCTTTTTGATGACTTCTAGCAAGCCTCGCTAACCTCGCCTTA890.10294491868508109No Hit
ACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAACT890.10294491868508109No Hit
ACCTTGTTGTTTATCGAAAGATATTCAAGGAGAAAGATCTGACTCTCCAA890.10294491868508109No Hit
GAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTA880.10178823420547342No Hit
GGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGAGTG870.10063154972586577No Hit
GCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTCTCTTCTCCAAGCA870.10063154972586577No Hit
ATTTTAGGTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCT870.10063154972586577No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)