FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86454
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAGTT5000.5783422398038263No Hit
ACATAACACATTGCATGCTACATAATACACTGTAACCTTCTAGCAGGGGT4780.552895181252458No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT3450.39905614546464013No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA3430.39674277650542483No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT3410.3944294075462096No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT3330.38517593170934833No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA3160.3655122955560182No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2970.3435352904434728No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC2940.34006523700464986No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT2940.34006523700464986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.3099914405348509No Hit
GAATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGC2630.30420801813681264No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA2580.2984245957387744No Hit
GTACATGGGAGGAGGCCAAGCGGCAGAGATTGCCAATGCCAAGTCCAGAG2520.29148448886112843No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT2500.28917111990191313No Hit
ATTTAATAGTTCAGACATCTTAGACAAGACTTGACTTTTGGGCTTCAGCA2410.2787609595854443No Hit
TCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTT2260.2614106923913295No Hit
GTATAACCCACTGCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTCT2250.2602540079117218No Hit
GAAGTAGGGTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATTC2230.2579406389525065No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC2060.23827700279917646No Hit
GAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGCACAGAGA2030.23480694936035348No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG2020.2336502648807458No Hit
TGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCCTT1990.23018021144192288No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT1990.23018021144192288No Hit
CCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTACTTGCCG1930.22324010456427698No Hit
CTACATAACACATTGCATGCTACATAATACACTGTAACCTTCTAGCAGGG1920.2220834200846693No Hit
TTCCAGGACTGGCTACTGCGTACATCCACAGGTTCACTAACGGGGGCAAG1900.219770051125454No Hit
GGATTATGATGCGACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAG1830.21167325976820042No Hit
TCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGGAAACTTTTT1820.21051657528859277No Hit
GCCTTATGTGGACCATTTTCTCAGACTGGAACGTGAGAAACGAGCCCGCA1770.20473315289055452No Hit
GGGTTATACCTTGGCACCTAAATAAAAGATACAATAGGAAACGATTCTTT1760.20357646841094684No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT1720.19894973049251627No Hit
ATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAGTTAT1690.1954796770536933No Hit
GGCCATAACTGAGTTATTTTTTTCATCAATCAGGAAAATGCTTCCTTAAT1630.18853957017604736No Hit
TCTTCAGACTGGTCAAGAAAGAGCTAGACCTGGTGCTTGGAGAAGAGACA1600.1850695167372244No Hit
AGATAAAGTTGTCCAGGAAAGAGAAGATGCCCTAAGGCTTCTTCAGACTG1550.17928609433918616No Hit
GGTATCAACGCAGAAGTAGGGTTTTGGCCTAGGTAACGGGGCAGAGATGT1530.17697272537997086No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1520.17581604090036318No Hit
TCTCTGGAGTTGATCGTTACTATGTGTCAAAGGGTTTGGAGAACATTGAT1510.17465935642075556No Hit
GTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA1510.17465935642075556No Hit
AGGTAACGGGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCA1490.17234598746154023No Hit
TCCTTAATCAATGTTCTCCAAACCCTTTGACACATAGTAACGATCAACTC1450.16771924954310963No Hit
GGTATCAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCT1350.1561524047470331No Hit
GACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGC1350.1561524047470331No Hit
GGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTCCGTCATGGGCGTG1340.15499572026742545No Hit
AATTTACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCACTC1340.15499572026742545No Hit
TCACTGGAGTCTGATGGAAAGAGATAGGCGCATCTCTGGAGTTGATCGTT1310.1515256668286025No Hit
GGATAGTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAA1310.1515256668286025No Hit
TTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTGAGCCAAC1300.15036898234899485No Hit
CTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTCTA1300.15036898234899485No Hit
ACATAAGGCAAGGCAAAGAATCGTTTCCTATTGTATCTTTTATTTAGGTG1300.15036898234899485No Hit
CCCCTACTCATCGCACTAATTTACACTCACAACACCCTAGGCTCACTAAA1290.1492122978693872No Hit
GTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCGGTG1290.1492122978693872No Hit
TACTAGCACAGAGAGTTCTCCCAGTAGGTTAATAGTGGGGGGTAAGGCGA1260.14574224443056422No Hit
CTACATAATACACTGTAACCTTCTAGCAGGGGTAGATGGCCATAACTGAG1260.14574224443056422No Hit
GTTCAGACATCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGG1250.14458555995095657No Hit
TTCCTACACTTTGCCTAATTGAGCCACTACCAGGTTTTCTGGCAGCTGTG1240.14342887547134894No Hit
GCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGC1230.14227219099174127No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT1220.14111550651213361No Hit
ATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACA1200.13880213755291831No Hit
CTATTGACCCAGCGATGGGGGCTTCGACATGGGCTTTAGGGAGTCATAAG1180.136488768593703No Hit
GTATCAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTT1150.13301871515488004No Hit
GTACATGGGAACCAAAATCGATATCAGCAATGTAAAAATCCCAAAACATC1110.12839197723644943No Hit
CTCCAGAGATGCGCCTATCTCTTTCCATCAGACTCCAGTGATACCCAAAA1110.12839197723644943No Hit
GCCCAAGAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTA1110.12839197723644943No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT1100.12723529275684178No Hit
GAAAAAAGGGTTGCTCATTTTGGGTATCACTGGAGTCTGATGGAAAGAGA1100.12723529275684178No Hit
ATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG1100.12723529275684178No Hit
GCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTC1090.12607860827723413No Hit
CTCCAAACCCTTTGACACATAGTAACGATCAACTCCAGAGATGCGCCTAT1090.12607860827723413No Hit
GGTATAACCCACTGCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTC1080.12492192379762648No Hit
CCTTGAGAGAGGATTATGATGCGACTGTGAGTGCGTTCGTAGTTTGAGTT1070.12376523931801883No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGA1070.12376523931801883No Hit
AGTATGTTGAGTCCTGTGAGTAGGAGAGTGATATTTGATCAGGAGAACGT1060.12260855483841118No Hit
CCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGT1050.12145187035880352No Hit
CAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATA1050.12145187035880352No Hit
GTCCAGAGCGGTTAGATAAGGTAGTAGATTCCATGGATGCATTAGATAAA1040.12029518587919588No Hit
CTATTAGTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGA1040.12029518587919588No Hit
GGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCAT1040.12029518587919588No Hit
CTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAA1030.11913850139958823No Hit
GCTTGAACTTGTGCCAGATGATTCTTCCAAAGATGTCTCTTCTCCAAGCA1020.11798181691998057No Hit
CTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCAC1020.11798181691998057No Hit
ACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATA1000.11566844796076527No Hit
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA1000.11566844796076527No Hit
ATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCAT990.11451176348115762No Hit
CCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTA990.11451176348115762No Hit
GTCCTTGAGAGAGGATTATGATGCGACTGTGAGTGCGTTCGTAGTTTGAG990.11451176348115762No Hit
ATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCG990.11451176348115762No Hit
GGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTC970.11219839452194232No Hit
CTCCTACTCACAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTA970.11219839452194232No Hit
GTACTATTGACCCAGCGATGGGGGCTTCGACATGGGCTTTAGGGAGTCAT970.11219839452194232No Hit
CCCTTCCCCTACTCATCGCACTAATTTACACTCACAACACCCTAGGCTCA970.11219839452194232No Hit
TATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTA960.11104171004233465No Hit
GGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTG960.11104171004233465No Hit
GGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATTCTCCCCGGACTCTC960.11104171004233465No Hit
ACTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTT950.109885025562727No Hit
GCGTACATCCACAGGTTCACTAACGGGGGCAAGGAAAAAAGGGTTGCTCA950.109885025562727No Hit
GCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTCTTCAATCAGC940.10872834108311934No Hit
GTATCAACGCAGAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGT940.10872834108311934No Hit
GTACATGGGAAGGAGAGAAAAACAAAAATGAGATTCCTACACTTTGCCTA930.1075716566035117No Hit
CATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGC920.10641497212390404No Hit
AGGTAGTAGATTCCATGGATGCATTAGATAAAGTTGTCCAGGAAAGAGAA910.10525828764429639No Hit
GTTTTAAGAGTACTGCGGCAAGTACTATTGACCCAGCGATGGGGGCTTCG910.10525828764429639No Hit
GGTCAAGAAAGAGCTAGACCTGGTGCTTGGAGAAGAGACATCTTTGGAAG900.10410160316468875No Hit
CACAACACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCA900.10410160316468875No Hit
CCCTAAGGCTTCTTCAGACTGGTCAAGAAAGAGCTAGACCTGGTGCTTGG890.10294491868508109No Hit
ATCTTAGACAAGACTTGACTTTTGGGCTTCAGCAAGATGTGGAAACTTTT880.10178823420547342No Hit
CTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAACAC880.10178823420547342No Hit
GTTTAAAGTGGCAATCTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCT870.10063154972586577No Hit
GGCTACTGCGTACATCCACAGGTTCACTAACGGGGGCAAGGAAAAAAGGG870.10063154972586577No Hit
GAGTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGAT870.10063154972586577No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)