FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299151
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT30931.0339260106100263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.6538503966224415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.5191358210402105No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT11140.3723871890784253RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.2757804586981157No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT7560.2527151839706369RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG6350.21226738336157994RNA PCR Primer, Index 46 (96% over 28bp)
TCTCTCTACTGATCAACAGACTACATTTTCACAACTCTTCTTTCCTTTAC5690.19020494666573068No Hit
GTATAAAGGCCCTCAATTGTAGTTGTTTCCAGCTGAATTAAGAGCTTTAA5270.17616521422291753No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA4750.15878268834133932No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4610.15410277752706827No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC4590.15343421883931527No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG4430.1480857493372912No Hit
CCCCATACCCAACCCCCTGGTTAACCTCAACCTAGGCCTCCTATTTATTC3890.13003466476796No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT3860.12903182673633048No Hit
TCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAA3620.12100912248329439No Hit
GTTCATAACTGCTTGTTGAAGCACTGTCTTGATGACTCCTTTAAAATTTG3540.11833488773228236No Hit
CTTTATTACCAAGCATCTTGGTCTCTCATTCAACAAGTACTGTATCTCAC3530.11800060838840584No Hit
CCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTC3510.11733204970065285No Hit
CTATAATTCCATGTTGTTTTCTCAGTTTCCAGTCAGAGAAGAGCTCTGCT3500.11699777035677635No Hit
GTGTTAACATGTTCATTATACAAGAGTGGGAATAAAAATGCTGAGATACA3500.11699777035677635No Hit
GTCTTGATGACTCCTTTAAAATTTGTGTCAGGGCGAGGTGCCATGACTCA3440.11499209429351731No Hit
ATCTAATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTAC3310.11064646282312277No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT3310.11064646282312277No Hit
CACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCC3160.1056322726649752No Hit
GAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAA3120.10429515528946919No Hit
ATACAAGAGTGGGAATAAAAATGCTGAGATACACAGAGGCATACCATCTA3080.10295803791396319No Hit
TTATACAAGAGTGGGAATAAAAATGCTGAGATACACAGAGGCATACCATC3060.10228947922621018No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)