FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001422000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299151
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGGCCCTCAATTGTAGTTGTTTCCAGCTGAATTAAGAGCTTTAA5710.19087350535348369No Hit
TCTCTCTACTGATCAACAGACTACATTTTCACAACTCTTCTTTCCTTTAC5520.18452219781983012No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG5290.17683377291067054No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA5040.16847678931375792No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA5040.16847678931375792No Hit
CCCCATACCCAACCCCCTGGTTAACCTCAACCTAGGCCTCCTATTTATTC4370.14608007327403216No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC4260.14240300049139062No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT4090.13672025164549007No Hit
GAATTAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAA3890.13003466476796No Hit
GTCTTGATGACTCCTTTAAAATTTGTGTCAGGGCGAGGTGCCATGACTCA3800.12702615067307146No Hit
ATCTAATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTAC3760.12568903329756545No Hit
CTATAATTCCATGTTGTTTTCTCAGTTTCCAGTCAGAGAAGAGCTCTGCT3500.11699777035677635No Hit
ATACAAGAGTGGGAATAAAAATGCTGAGATACACAGAGGCATACCATCTA3460.11566065298127035No Hit
GTGTTAACATGTTCATTATACAAGAGTGGGAATAAAAATGCTGAGATACA3400.1136549769180113No Hit
CCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTC3380.11298641823025829No Hit
TCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAA3350.1119835801986288No Hit
ATAATAATGTTTGTGTATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGC3270.10930934544761675No Hit
CACAATATCTTACTTGATCCTCACAACAACCCTGTGAGGTAGGTGTTAAC3260.10897506610374026No Hit
GTTCATAACTGCTTGTTGAAGCACTGTCTTGATGACTCCTTTAAAATTTG3190.10663511069660472No Hit
CTTTATTACCAAGCATCTTGGTCTCTCATTCAACAAGTACTGTATCTCAC3170.10596655200885172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.10529799332109871No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)