FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001422001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566504
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT211800.825247106569871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205090.7991025924759907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142930.5569054246554846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114220.4450411922210135No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT87840.34225545722897766RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT53110.20693519277585384RNA PCR Primer, Index 17 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.1200076056768273Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.10087652308354088No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)