FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001422003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180685
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT158388.765531173035948No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT77624.295874034922655RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT52342.896754019426073RNA PCR Primer, Index 26 (100% over 24bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG15160.8390292497993745RNA PCR Primer, Index 26 (96% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG14130.7820239643578604RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT13650.7554583944433684RNA PCR Primer, Index 26 (100% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.7305531726485319No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC11480.6353598804549354RNA PCR Primer, Index 26 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.5728201012812353No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT8830.4886957965520104RNA PCR Primer, Index 26 (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.466557821623267No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG4670.2584608572930791No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC4420.2446246229626145RNA PCR Primer, Index 26 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.21639870492846666Clontech SMART CDS Primer II A (100% over 21bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA3740.20699006558375072RNA PCR Primer, Index 26 (100% over 29bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2520.13946924205108338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.1383623433046462No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2020.11179677339015415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)