FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001422015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910334
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT146501.6092994439403558No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT65620.7208343311356052TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT44050.4838883310960593RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.41742920730193533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37430.41116776919240633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.25891595831859515No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG15200.16697168292077413TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT14890.16356633938752152TruSeq Adapter, Index 10 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.1404978831945198Clontech SMART CDS Primer II A (100% over 21bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG12260.13467584425057175TruSeq Adapter, Index 10 (95% over 24bp)
TCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATAATGGAA11760.12918335468080944No Hit
TCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATACACGTACTCCTGGACCC11340.12456966344220911No Hit
GTCCAGGAGTACGTGTATGACCTGGAACTGAACTGAGCTGCTCAGAGACA10850.11918702366384207No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG10720.11775897637570387No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC9600.10545579973943629No Hit
CTCCTGGACCCAGGACTCACTGGGGTCAGCGCAGACTTGCTTGCCTCTTT9190.10095195829223119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)