FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001422061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182071
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT79174.348303683727776No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT66823.669996869353164RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT51822.8461424389386556RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23081.2676373502644573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.9326032152292237No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG14550.7991387975020734TruSeq Adapter, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.7370750970775137No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT10050.5519824683777208RNA PCR Primer, Index 17 (96% over 26bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG10030.5508839958038348RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC6850.37622685655595894No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT6330.34766656963492265No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.32789406330497445No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.291644468366736No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG5300.29109523207979304RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG4180.2295807679421764RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG4120.22628535022051838RNA PCR Primer, Index 33 (96% over 29bp)
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC4000.2196945147772023No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA3750.20596360760362717RNA PCR Primer, Index 17 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.20486513502974116No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC3520.193331173003938TruSeq Adapter, Index 25 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA3030.16641859494373076No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA2770.1521384514832126RNA PCR Primer, Index 31 (96% over 29bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.14774456118766854Clontech SMART CDS Primer II A (100% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC2180.11973351055357526No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA2020.11094572996248717RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1920.1054533670930571RNA PCR Primer, Index 33 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.10490413080611409No Hit
CTCTAGGACAATCTTTCTTCTAAAACCTCCATTCTTTCATCCCATTCACT1860.10215794937139908No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)