FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001422065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681616
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT227353.3354557404755756No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT185202.7170723691932115RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT133601.9600478861998543RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115671.6969965493767751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75881.1132367784793784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60630.8895037675171944No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG35010.5136323091007253RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC20290.2976749372080467No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT17580.25791648083378327RNA PCR Primer, Index 27 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.2137567193258374No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.19541794793549444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.17722588671627426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.17150419004248726Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10930.16035421703715877No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC9660.14172202530457031RNA PCR Primer, Index 27 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT9110.13365296589281941RNA PCR Primer, Index 27 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG9040.1326259946949602RNA PCR Primer, Index 27 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8020.11766155724044036No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC7320.10739184526184832No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)