FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001422079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1449.842788790157211RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG926.288448393711551RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG886.015037593984962RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG473.2125768967874233RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA473.2125768967874233RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT372.52904989747095RNA PCR Primer, Index 31 (96% over 27bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG352.3923444976076556RNA PCR Primer, Index 39 (96% over 30bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA342.3239917976760083RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG342.3239917976760083TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA322.1872863978127137RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT271.845522898154477RNA PCR Primer, Index 27 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG261.77717019822283TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG231.5721120984278878TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA221.5037593984962405RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA211.4354066985645932RNA PCR Primer, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA181.2303485987696514RNA PCR Primer, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG140.9569377990430622RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA120.8202323991797676RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT110.7518796992481203RNA PCR Primer, Index 33 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT100.683526999316473RNA PCR Primer, Index 31 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.683526999316473No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG100.683526999316473RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA90.6151742993848257RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG90.6151742993848257RNA PCR Primer, Index 26 (100% over 29bp)
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA80.5468215994531784RNA PCR Primer, Index 27 (100% over 28bp)
GAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA80.5468215994531784RNA PCR Primer, Index 38 (96% over 28bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA80.5468215994531784RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA80.5468215994531784RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTTCTGCTTG70.4784688995215311RNA PCR Primer, Index 38 (96% over 27bp)
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA70.4784688995215311TruSeq Adapter, Index 11 (96% over 28bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC60.4101161995898838RNA PCR Primer, Index 38 (96% over 25bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC60.4101161995898838TruSeq Adapter, Index 27 (96% over 25bp)
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA60.4101161995898838RNA PCR Primer, Index 38 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT50.3417634996582365RNA PCR Primer, Index 17 (96% over 26bp)
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA50.3417634996582365Illumina PCR Primer Index 1 (96% over 27bp)
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT50.3417634996582365RNA PCR Primer, Index 27 (100% over 27bp)
TCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA50.3417634996582365RNA PCR Primer, Index 15 (96% over 28bp)
GAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAA50.3417634996582365RNA PCR Primer, Index 17 (96% over 27bp)
GAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAA50.3417634996582365RNA PCR Primer, Index 15 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG40.2734107997265892RNA PCR Primer, Index 27 (100% over 24bp)
GAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA40.2734107997265892TruSeq Adapter, Index 27 (96% over 28bp)
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA40.2734107997265892Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT40.2734107997265892RNA PCR Primer, Index 31 (95% over 22bp)
TCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTTCTGCTTG40.2734107997265892RNA PCR Primer, Index 38 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2734107997265892No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA40.2734107997265892RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCTGCTTG30.2050580997949419Illumina PCR Primer Index 4 (96% over 26bp)
CCATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC30.2050580997949419TruSeq Adapter, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCTGCTTG30.2050580997949419RNA PCR Primer, Index 38 (96% over 26bp)
GAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA30.2050580997949419Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT30.2050580997949419TruSeq Adapter, Index 1 (96% over 26bp)
TCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGTCTTCTGCTTG30.2050580997949419TruSeq Adapter, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG30.2050580997949419RNA PCR Primer, Index 38 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT30.2050580997949419RNA PCR Primer, Index 27 (96% over 25bp)
ATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT30.2050580997949419RNA PCR Primer, Index 38 (96% over 27bp)
CTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGTCTTCTGCTTGA20.1367053998632946RNA PCR Primer, Index 11 (96% over 28bp)
GCTCGGAGATGTGTATAAGAGACAGGTCTCCGAGCCCACGAGACGCTACG20.1367053998632946No Hit
GAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1367053998632946Illumina PCR Primer Index 1 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT20.1367053998632946TruSeq Adapter, Index 11 (96% over 26bp)
TCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA20.1367053998632946TruSeq Adapter, Index 12 (96% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCTGCTTG20.1367053998632946RNA PCR Primer, Index 26 (96% over 29bp)
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC20.1367053998632946Illumina PCR Primer Index 5 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGTCTTCTGCTTG20.1367053998632946RNA PCR Primer, Index 38 (96% over 27bp)
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA20.1367053998632946TruSeq Adapter, Index 4 (96% over 27bp)
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA20.1367053998632946TruSeq Adapter, Index 10 (96% over 28bp)
CTCGGAGATGTGTATAAGAGACAGATCTCCGAGCCCACGAGACAAGAGGC20.1367053998632946No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT20.1367053998632946RNA PCR Primer, Index 15 (96% over 27bp)
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTG20.1367053998632946Illumina PCR Primer Index 9 (96% over 26bp)
TCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCTGCTTG20.1367053998632946RNA PCR Primer, Index 38 (96% over 27bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCCTGA20.1367053998632946RNA PCR Primer, Index 27 (96% over 27bp)
GAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1367053998632946TruSeq Adapter, Index 25 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGGACCAGAGAGGATCTCGTATGCCGTC20.1367053998632946RNA PCR Primer, Index 23 (95% over 21bp)
CTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTTCTGCTTGA20.1367053998632946RNA PCR Primer, Index 38 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT20.1367053998632946No Hit
GGCTCGGAGATGTGTATAAGAGACAGACTCCGAGCCCACGAGACAAGAGG20.1367053998632946No Hit
CTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTTCTGCTTGA20.1367053998632946Illumina PCR Primer Index 5 (96% over 26bp)
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA20.1367053998632946TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGTCTTCTGCTTGA20.1367053998632946RNA PCR Primer, Index 19 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1367053998632946No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG20.1367053998632946Illumina PCR Primer Index 4 (96% over 26bp)
CTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTGA20.1367053998632946Illumina PCR Primer Index 4 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)