FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001422080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001422080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT120.8202323991797676Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG80.5468215994531784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.5468215994531784No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT60.4101161995898838Illumina RNA PCR Primer (100% over 25bp)
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT60.4101161995898838Illumina Single End PCR Primer 1 (96% over 26bp)
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT40.2734107997265892Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG40.2734107997265892No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG40.2734107997265892No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG30.2050580997949419No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT30.2050580997949419Illumina Single End PCR Primer 1 (95% over 24bp)
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC30.2050580997949419No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG30.2050580997949419No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC20.1367053998632946No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCTCCGT20.1367053998632946No Hit
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG20.1367053998632946No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC20.1367053998632946Illumina Single End PCR Primer 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)