FastQCFastQC Report
Fri 24 May 2024
EGAF00001427089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001427089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149771313
Total Bases11.3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC21244371.4184538797493216No Hit
ACTATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT19082731.2741245047374328No Hit
CTTATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT18752951.2521056018250971No Hit
AGCATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT15894961.0612820093257778No Hit
TGTATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT15384191.0271786827428027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14974670.9998356627881069No Hit
TACATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT13516200.902455866164437No Hit
TCGATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT13468030.8992396294208891No Hit
GCAATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC11121940.7425948118649397No Hit
CTTGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT10755240.7181108173899764No Hit
CAAATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC10673540.7126558341649847No Hit
AGCGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT10484820.7000552902944771No Hit
TACATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC9998410.6675784434099206No Hit
TCGGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT9388800.6268757221885343No Hit
ATGATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT9313540.6218507278493312No Hit
CAAATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT9134870.6099212070071123No Hit
GCAATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT8956160.5979890154264722No Hit
TGTGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT8122230.5423087931398451No Hit
ACTGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT7973290.5323642986290706No Hit
CTTCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT7967750.5319944013577553No Hit
GCAGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT7258750.48465556284466843No Hit
TACGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT7061350.4714754687367934No Hit
AGCATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC6671430.4454411106084114No Hit
TCGATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC6570830.43872420347947405No Hit
GAGGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT6318420.421871176358052No Hit
GAGATTTGTGAATTGGGGTGATCGAAAGAAAAATGCACAGGTGGTGAACT6311270.42139378186528953No Hit
GAGATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC5417940.3617475130234052No Hit
TGTCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT5395970.36028060994564426No Hit
CAAGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT4201220.280508991731948No Hit
ATGGTGTTTTCTGTCAGGGGATCATAAAATAACTCTGGTTTTACTCTTTT3934180.2626791420330274No Hit
ACTATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC3921160.26180981667697606No Hit
ACTCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT3529000.23562589719701527No Hit
TGTATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC3153630.2105630201692897No Hit
CTTGTGTTTTCTGTCAGGGGATCGTAAAATAACTCTGGTTTTACTCTTTT2890910.1930216102198423No Hit
CTTATTTGTGAATTGGGGTGATCAAGAACAAAAAATAAACTATCAGATTC2638260.1761525586678939No Hit
AGCCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT2353000.1571061876181856No Hit
ATGCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT2188320.1461107575387284No Hit
CAACAAAGAGGGTGTTAATGTAGATCATGGGAAAGGCAGCAGAGCCAGAG2183160.14576623228241312No Hit
TACCAAAGAGGGTGTTAATGTAGATCATGGGAAAGGCAGCAGAGCCAGAG2050890.13693476800861057No Hit
CAACAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT2049540.13684463058690016No Hit
ACTCAAAGAGGGTGTTAATGTAGATCGGAAGAGCGGTTCAGCAGGAATGC2039240.13615691544348016Illumina Paired End PCR Primer 2 (100% over 29bp)
TACCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT1957070.130670551042041No Hit
ATGGTGTTTTCTGTCAGGGGATCATGTTTTCCTGTAAGTACAAAGCTTCT1910200.1275411132971773No Hit
TCGCAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT1826580.1219579346279751No Hit
GCACAAAGAGGGTGTTAATGTAGATCCTGGCAAACTGGAAGCAGCAAAGT1539740.10280606941063539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)