FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001432543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33031070
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18000645.449608504962146No Hit
TGCATCAGCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9892322.9948530277705205No Hit
TGCATCAGCGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC7396232.239173602308372No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1730220.5238159102929454TruSeq Adapter, Index 13 (97% over 37bp)
TGCATCAGCGCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC346600.10493150842524931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)