FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001432544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33031070
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16127474.88251515921222No Hit
TGCATCAGCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9895152.9957097968670103No Hit
TGCATCAGCGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6127501.8550716037960622No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC2050210.6206913672490779Illumina Single End PCR Primer 1 (96% over 33bp)
TGCATCAGCGCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC370370.1121277633452383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)