FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27107433
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT13972315.154420191686907No Hit
TGCATCAGCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8636133.185890010315621No Hit
TGCATCAGCGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5053741.8643373572112123No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC1450040.5349233916763716Illumina Single End PCR Primer 1 (96% over 33bp)
TGCATCAGCGCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC303270.11187706338700533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)