FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23597327
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATATGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT10120154.288684900624549No Hit
AGCCATATGAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6272762.6582502331725966No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC5399352.2881193280916943Illumina Single End PCR Primer 1 (96% over 33bp)
AGCCATATGAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC3680851.5598588772363922No Hit
AGCCATATGAGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC388510.1646415291020038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)