FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22327038
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCCCTGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9599194.299356681347522No Hit
CCTGCCCCTGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6206502.77981342621444No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3426041.534480301417501Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGCCCCTGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2042490.9148056271503635No Hit
CCTGCCCCTGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1813930.8124364727645467No Hit
CCTGCCCCTGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGC275250.12328101918400462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)