FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001432555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28333651
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGTCATGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12365164.364125188102303No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG10513183.7104925164780216TruSeq Adapter, Index 3 (97% over 35bp)
TAAAGTCATGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8178552.8865146958999386No Hit
TAAAGTCATGCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2216220.782186524426379No Hit
TAAAGTCATGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1943960.6860958370666738No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG809680.2857662078212229TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCACCTCGTATG387180.13665023261562728TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTATG286360.10106710215354879TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)