FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001432557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37930908
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGTCATGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16674684.396066658884095No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG11663203.0748538896036974TruSeq Adapter, Index 3 (97% over 35bp)
TAAAGTCATGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA11321912.984877135026665No Hit
TAAAGTCATGCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2986390.7873236253664163No Hit
TAAAGTCATGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2646900.6978214178263278No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG738400.19466974004418772TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCACCTCGTATG437810.11542302124694721TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)