FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001432559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19265495
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTCAGAAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9847275.1113506297139No Hit
AGAACTCAGAATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6380873.3120716597211755No Hit
AGAACTCAGAAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC3352191.7399968181456018No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG3033591.5746234394704108TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTATG264090.13707927047812682TruSeq Adapter, Index 7 (97% over 35bp)
AGAACTCAGAAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC235310.12214064575034277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)