FastQCFastQC Report
Fri 31 Mar 2023
EGAF00001432560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19265495
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTCAGAAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8981144.661774846688341No Hit
AGAACTCAGAATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6304813.272591750173043No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3381071.7549873491441565Illumina Single End PCR Primer 1 (96% over 33bp)
AGAACTCAGAAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2936821.524393741245683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)