FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25186471
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTCAGAAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12920215.129821482334703No Hit
AGAACTCAGAATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8502013.37562574764841No Hit
AGAACTCAGAAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC4431421.759444584356419No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG3221591.2790954318292547TruSeq Adapter, Index 7 (97% over 35bp)
AGAACTCAGAAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC305960.12147791566353222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)