FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25186471
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTCAGAAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12256494.866299053964329No Hit
AGAACTCAGAATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8472563.3639329622637484No Hit
AGAACTCAGAAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC3894171.546135621778851No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3680321.4612289272284316Illumina Single End PCR Primer 1 (96% over 33bp)
AGAACTCAGAAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC256310.10176495150908597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)