FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26538602
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9189713.462770947768839No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6138142.312910077177389No Hit
TCACCTGTCGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2368490.892469769131019No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG2078250.783104550872725TruSeq Adapter, Index 8 (97% over 35bp)
TCACCTGTCGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC939920.3541708790839849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)