FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001432568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26538602
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8612753.2453668810436964No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA5318212.0039525819785085No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC2275190.8573134334657115Illumina Single End PCR Primer 1 (96% over 33bp)
TCACCTGTCGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1994230.7514450082939561No Hit
TCACCTGTCGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC875790.3300060794460839No Hit
CACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA301910.11376258628845634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)