FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001432569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30321468
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT10670773.519212856053012No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA7262292.3950984167389255No Hit
TCACCTGTCGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2719760.8969750409182036No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG1972360.6504830175108935TruSeq Adapter, Index 8 (97% over 35bp)
TCACCTGTCGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1078030.35553357772783295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)