FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001432571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44010032
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTGATACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT17170803.9015649886371366No Hit
ATAGTGATACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA10620332.4131611628912246No Hit
ATAGTGATACTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC6130121.392891511644436No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG4423451.0051003825673201TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATG519800.1181094346852554TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)