FastQCFastQC Report
Sun 14 May 2023
EGAF00001432576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43822941
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGCGAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT7004551.5983751524116103No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC6246421.425376722205842Illumina Single End PCR Primer 1 (96% over 31bp)
CATCAGCGAACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA4312200.9840051583940931No Hit
CATCAGCGAACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2094820.47801903573746907No Hit
CATCAGCGAACGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC565130.12895757041956632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)