FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001432578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38334803
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGCGAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT6422191.6752896838937714No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC4538271.183851133916092Illumina Single End PCR Primer 1 (96% over 31bp)
CATCAGCGAACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3932681.0258771904997137No Hit
CATCAGCGAACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1861110.48548834332082No Hit
CATCAGCGAACGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC497240.1297098096473849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)